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Image Search Results
Journal: Journal of Bacteriology
Article Title: The Escherichia coli mqsR and ygiT Genes Encode a New Toxin-Antitoxin Pair
doi: 10.1128/JB.01266-09
Figure Lengend Snippet: MqsR does not inhibit protein synthesis in vitro. Coupled transcription-translation was carried out using the E. coli T7 S30 extract system for circular DNA. Templates for expression of β-lactamase as a positive control (pGEM9Zf) (lanes 2 and 5), MqsR (pTX4) (lanes 4 to 6), and YgiT plus LacI (pAT3) (lanes 3 and 6) were added. In vitro-synthesized proteins were labeled with [35S]methionine and analyzed by SDS-PAGE using a 14% polyacrylamide gel. The positions of synthesized proteins are indicated on the right.
Article Snippet: In vitro coupled transcription-translation was performed using the E. coli T7 S30 extract system for
Techniques: In Vitro, Expressing, Positive Control, Synthesized, Labeling, SDS Page